Molecular mechanisms controlling the phenotype and the EMT/MET dynamics of hepatocyte

The complex spatial and paracrine relationships between the various liver histotypes are essential for proper functioning of the hepatic parenchymal cells. Only within a correct tissue organization, in fact, they stably maintain their identity and differentiated phenotype. The loss of histotype identity, which invariably occurs in the primary hepatocytes in culture, or in vivo in particular pathological conditions (fibrosis and tumors), is mainly due to the phenomenon of epithelial-to-mesenchymal transition (EMT). The EMT process, that occurs in the many epithelial cells, appears to be driven by a number of general, non- tissue-specific, master transcriptional regulators. The reverse process, the mesenchymal-to epithelial transition (MET), as yet much less characterized at a molecular level, restores specific epithelial identities, and thus, must include tissue-specific master elements. In this review, we will summarize the so far unveiled events of EMT/MET occurring in liver cells. In particular, we will focus on hepatocyte and describe the pivotal role in the control of EMT/MET dynamics exerted by a tissue-specific molecular mini-circuitry. Recent evidence, indeed, highlighted as two transcriptional factors, the master gene of EMT Snail, and the master gene of hepatocyte differentiation HNF4α, exhorting a direct reciprocal repression, act as pivotal elements in determining opposite cellular outcomes. The different balances between these two master regulators, further integrated by specific microRNAs, in fact, were found responsible for the EMT/METs dynamics as well as for the preservation of both hepatocyte and stem/precursor cells identity and differentiation. Overall these findings impact the maintenance of stem cells and differentiated cells both in in vivo EMT/MET physio-pathological processes as well as in culture.The complex spatial and paracrine relationships between the various liver histotypes are essential for proper functioning of the hepatic parenchymal cells. Only within a correct tissue organization, in fact, they stably maintain their identity and differentiated phenotype. The loss of histotype identity, which invariably occurs in the primary hepatocytes in culture, or in vivo in particular pathological conditions (fibrosis and tumors), is mainly due to the phenomenon of epithelial-to-mesenchymal transition (EMT). The EMT process, that occurs in the many epithelial cells, appears to be driven by a number of general, non- tissue-specific, master transcriptional regulators. The reverse process, the mesenchymal-to epithelial transition (MET), as yet much less characterized at a molecular level, restores specific epithelial identities, and thus, must include tissue-specific master elements. In this review, we will summarize the so far unveiled events of EMT/MET occurring in liver cells. I

Design Dynamics. Navigating the new Complex Landscape of Omnichannel Fashion Retail

The fashion industry is entering the dynamic global competitive market, promoting various actions prioritising design, creativity, sustainability, and technological advancement as pivotal factors. At the same time, it is reimagining its business models to adapt to the changing landscape. The rise of pervasive connectivity, intuitive interfaces and innovative interaction channels has triggered a revolution in fashion retail, reshaping customer behaviour and expectations. The traditional retail framework has evolved into a fully interconnected omnichannel system. This transformation is characterised by the proliferation of physical and virtual channels and touch points and by the adoption of a more flexible and integrated approach. In this dynamic context, design plays a central role, possessing the ability to impart meaning to the production and distribution system. Design-led innovation represents an incremental form of innovation that injects a nuanced range of meaning into the marketplace, extending beyond tangible objects, including discourses, expressions, narratives, visual images, symbols, metaphors, and spaces. The book analyses the multifaceted nature of the fashion retail experience through the lens of the design discipline, aiming to contextualise the evolution of retail within increasingly complex processes, networks and interconnections, both theoretically and practically. The focus is on retail design, delving into the new skills required and the valuable tools needed to apply them in inherently multidisciplinary contexts. Ultimately, the aim is to navigate the intricate terrain of retail evolution and shed light on the evolving role of design in this multifaceted sector

Psychometric properties of a Portuguese version of the Subjective Happiness Scale

The Subjective Happiness Scale (SHS) constitutes an instrument for assessing subjective happiness. This study aims to present the validation of the SHS in a Portuguese adult population. A large representative sample (1,017 participants), from five different age groups was considered. Configurational invariance of the unidimensional structure of the scale was tested through multi-group confirmatory factorial analysis, and reliability and criterion-validity were investigated. The results support the single factorial structure and reliability of the SHS. Furthermore, the SHS correlated positively with the Satisfaction with Life Scale, thus supporting its criterion validity. Therefore, the Portuguese version of the SHS is a valid instrument to use with an adult Portuguese Sample.info:eu-repo/semantics/publishedVersio

A fracture mechanics model to study indentation cutting

Many cutting processes, such as chopping, slicing, and carving, consist in 2 different stages: an initial stage of indentation, in which the cutting tool is pushed into the material under the action of an external force, and a second stage, where the target material undergoes a progressive separation. This second stage is characterised by the formation of a fracture surface followed by the cut propagation due to the increasing external force, until eventually a steady state might occur. The purpose of this paper is to analyse the cutting process by means of some concepts of fracture mechanics and discuss the occurrence of the steady state. A simple model is used to obtain an analytic expression of the stress intensity factor at the tip of the cut and investigate the evolution of the fracture process. It is found that the cut propagation depends on the wedge sharpness. The analytic results are compared with finite element analyses, where the effect of tip blunting due to plasticity is taken into account. The influence of the cutting tool geometry is also discussed

full model multiaxial fatigue life calculations with different criteria

Abstract Fatigue design of structural components under multiaxial loading often relies on fatigue life calculations based on a post-processing of the full-field elastic stress/strain maps obtained from finite element (FE) analysis. In the present article, a post-processing tool is adopted to compute the fatigue life of a structural component, and multiaxial fatigue assessment is carried out by considering different criteria such as that of Smith-Watson-Topper and of Fatemi-Socie. The present paper focuses on a specific structural component related to a quarter-turn heavy-duty valve actuator, called scotch yoke, commonly used in many application sectors such as oil & gas, power and chemical industries. The fatigue assessment of the component is carried out by employing a full-model FE analysis, considering fillet-welded joints exposed to in-phase constant amplitude cyclic bending-torsion fatigue load with load ratio R=-1, with applied maximum load according to EN 15714-3, which is the standard in the valve actuator sector. The elastic stress/strain field extracted from the FE model is used to perform the fatigue assessment of the fillet-welded joints, where the potential fatigue crack initiation points (weld toe and weld root) are described by adopting an effective radius at the weld notches

on the fracture processes of cutting

Abstract: The process of cutting is treated as a fracture mechanical process. For an elliptic rigid wedge pressed into an elastic material, fracture may occur as an autonomous process if the tip of the wedge is sufficiently blunt or is affected by the geometry of the wedge if the tip is sharp. The conditions leading to the former or the latter case is obtained as a relation between the wedge tip radius, the fracture toughness and the modulus of elasticity. These limits and the intermediate states are discussed. The implications of the drastic changes of the mechanical state of the near tip region when the wedge edge is sharp are also discussed

Entrepreneurial self-efficacy in Italy: an empirical study from a gender perspective

Entrepreneurial self-efficacy (ESE) is a key factor for the promotion of entrepreneurship. Literature emphasizes the need for and, indeed, recommends a gender perspective in the study of ESE. The aim of this study is twofold: To begin analyzing some of the psychometric properties of the McGee, Peterson, Mueller, and Sequeira (2009) ESE scale in the Italian context, and to explore the gender differences in the ESE levels in an Italian sample of entrepreneurs and non-entrepreneurs. Confirmatory factor analysis, multigroup confirmatory factor analysis and a 2 × 2 MANOVA were conducted. The results support the possible use of McGee et al.'s ESE scale in the Italian context. Furthermore, they reveal that the gender difference in the sample of non-entrepreneurs is greater than in the entrepreneur sample. The main practical implication is that women entrepreneurs should receive specific training both before and after creating a new venture.info:eu-repo/semantics/publishedVersio

Influence of Acute Resistance Training on Memory, Executive Function, and Mood

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Evaluation of the safety factors of shotcrete linings during the creep stage

Sprayed concrete linings (SCLs) used in tunnels generally develop secondary deformations over time, even in the presence of constant stress levels within the lining. These deformations influence the loading process on the lining and therefore also the stress levels within the support structure. The behaviour of a SCL in a tunnel was investigated under different operating conditions in order to evaluate the effect of secondary deformations on the evolution of stability conditions (safety margins with respect to the possible concrete failure) over time, after completion of the tunnel construction. A parametric analysis was performed to study eight different types of tunnels, with variable geometry and rock quality, and eight different types of sprayed concrete. The 64 cases of the parametric analysis covered the vast range of variability of influential parameters and yielded useful information concerning the effects of secondary deformations over time on the static behaviour of the lining and on the safety factor with reference to the possible failure of the sprayed concrete